Anti-SEC23 antibody [JU37-48]

产品描述

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.

产品名称Anti-SEC23 antibody [JU37-48]

分子量86/63 kDa

种属反应性Human,Mouse

验证应用WB,ICC,IHC-P,FC

抗体类型重组兔单抗

免疫原Recombinant protein

偶联Non-conjugated

性能

形态Liquid

浓度1 mg/mL.

存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型IgG

纯化方式Protein A affinity purified.

亚细胞定位Cytosol. Endoplasmic reticulum.

数据链接Q15436 Human

Q01405 Mouse

其它名称

CLSD antibody

Protein transport protein Sec23A antibody

SC23A_HUMAN antibody

more

应用

• WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

• 

  Fig1: Western blot analysis of SEC23 on NIH-3T3 cell using anti-SEC23 antibody at 1/500 dilution.

  

  Fig2: ICC staining SEC23 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig3: ICC staining SEC23 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SEC23 antibody. Counter stained with hematoxylin.

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SEC23 antibody. Counter stained with hematoxylin.

  

  Fig6: Flow cytometric analysis of HepG2 cells with SEC23 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Goat anti rabbit IgG (FITC) was used as the secondary antibody.