Anti-ACE2 antibody [SN0754] 

产品描述

• Angiotensin-converting enzyme (ACE) is a carboxyl-terminal dipeptidyl exopeptidase that converts angiotensin I to the potent vasopressive hormone, angiotensin II. There are two isoforms of ACE, the pulmonary ACEP and the testicular ACET. ACEP is a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney and spleen. The ACET isoform is expressed exclusively in adult testis by developing sperm cells, specifically late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, and is involved in blood pressure regulation and fluid/electrolyte homeostasis. ACE2 is the first known human homolog of ACE. Unlike ACE, which is expressed ubiquitously throughout the vasculature, ACE2 is expressed only in cardiac, renal and testicular cells.

• 产品名称Anti-ACE2 antibody [SN0754]

• 分子量92 kDa

• 种属反应性Human, Mouse, Hamster, Rat

• 验证应用WB, ICC, IHC-P, IP

• 抗体类型重组兔单抗

• 免疫原Synthetic peptide within human ACE2 aa 190-230.

• 偶联Non-conjugated

性能

形态Liquid

浓度1 mg/mL.

存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型IgG

纯化方式Protein A affinity purified.

亚细胞定位Secreted, Cell membrane, Cytoplasm.

数据链接SwissProt: Q9BYF1 Human

SwissProt: Q8R0I0 Mouse

SwissProt: Q5EGZ1 Rat

SwissProt: A0A1U7QTA1 Hamster

其它名称

ACE 2 antibody

ACE related carboxypeptidase antibody

ACE-related carboxypeptidase antibody

more

应用

• WB: 1:1,000-1:5,000
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200
  IP: Use at an assay dependent concentration.

• 

  Fig1: Western blot analysis of ACE2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: human kidney tissue lysate
  Lane 2: human small intestine tissue lysate

  

  Fig2: Western blot analysis of ACE2 on Hamster testis (1) and stomach (2) tissue lysates using anti-ACE2 antibody at 1/1,000 dilution.

  

  Fig3: ICC staining of ACE2 in 293 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibodyfor 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig4: ICC staining of ACE2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig5: ICC staining of ACE2 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ACE2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ACE2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.