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钙调素结合树脂
| 英文名 | Calmodulin Resin (Settled Resin) | ||
| 相关类别 | 亲和纯化介质 | 储存 | 冷藏（2-8℃） |
| 无 | 钙调素结合树脂 | ||
产品描述
概述
Calmodulin Resin for the affinity purification of calmodulin binding proteins (CBP), including recombinant proteins with a CBP tag and calmodulin-regulated proteins in eukaryotic cells. The resin is 4% agarose coupled to calmodulin and has a ligand density of approximately 1 mg calmodulin/ml resin.
特点
This resin is ideal for the purification of calmodulin binding proteins that are involved in many biological pathways, including glycogen metabolism,neurotransmission and cytoskeletal control. In addition, a growing use is the isolation of recombinant proteins that are fused to the calmodulin‐binding peptide (CBP).
应用
For the purification of calmodulin binding proteins and binds CBP tagged recombinant proteins
组份
10 ml/bottle
适用样本
Common lysis buffers are compatible with the resin, but must contain 2mM CaCl2.
技术规格
| Binding capacity: | 1-3 mg/ml (approx) |
| Bead size | 50~160 μm |
| Bead Structure | 4% highly cross-linked agarose |
| Ligand density | 0.9~1.2 mg calmodulin/ml resin |
| List is the maximum compatible levels of some common reagents | 50‐300 mM NaCl/ KCl/ NH3SO4, 5mM DTT, 10mM β‐mercaptoeth |