重组人UbcH5d, N-GST, E.coli
英文名 | Recombinant Human Ubiquitin-Conjugating Enzyme E2 D4 , N-GST, E.coli | ||
相关类别 | 其他重组蛋白 | 储存 | 冷冻(-20℃) |
产品描述
概述
Recombinant Human Ubiquitin-Conjugating Enzyme E2 D4 is produced by our E.coli expression system and the target gene encoding Met1-Met147 is expressed with a GST tag at the N-terminus.
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技术规格
Tag | N-GST |
种属 | Human |
表达系统 | E.coli |
Accession# | Q9Y2X8 |
Source | E.coli |
Formulation_Description | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
Storage | Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
Background | Ubiquitin-Conjugating Enzyme E2 D4 (UBE2D4) is a ligase that belongs to the Ubiquitin-Conjugating Enzyme family. UBE2D4 has been proposed to participate in Ubl conjugation pathway. UBE2D4 takes part in post-translational protein modification, protein K6-linked ubiquitination, protein K11-linked ubiquitination, protein K27-linked ubiquitination, protein K29-linked ubiquitination, protein K48-linked ubiquitination, and protein K63-linked ubiquitination. UBE2D4 regulate of protein metabolic process. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, UBE2D4 able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked poly-ubiquitination. |