重组人PPM1A, C-6His, E.coli
英文名 | Recombinant Human Protein Phosphatase 1A , C-6His, E.coli | ||
相关类别 | 其他重组蛋白 | 储存 | 冷冻(-20℃) |
产品描述
概述
Recombinant Human Protein Phosphatase 1A is produced by our E.coli expression system and the target gene encoding Gly2-Trp382 is expressed with a 6His tag at the C-terminus.
使用说明
This material is offered by Sangon Biotech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
技术规格
Tag | C-6His |
种属 | Human |
表达系统 | E.coli |
Accession# | P35813 |
Source | E.coli |
Formulation_Description | Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Storage | Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles. |
Purity | Greater than 90% as determined by reducing SDS-PAGE. |
Endotoxin | Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test. |
Background | Protein Phosphatase 1A (PPM1A) is a member of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1A has a broad specificity. PPM1A negatively regulates the activities of MAP kinases and MAP kinase kinases. Also, it negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. In addition, PPM1A can dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. |